Journal: Scientific Reports

Article Title: Persistence of Long-lived Memory B Cells specific to Duffy Binding Protein in individuals exposed to Plasmodium vivax

doi: 10.1038/s41598-018-26677-x

Figure Lengend Snippet: Study design. Schematic shown a flow work of three cross-sectional groups used in this study. Each dashed line refers to the sample which was taken for further experiment ( a ) subjects who had completely follow up at all time points of study, ( b ) subjects who had PBMCs available for ELISPOT assay at 9 m post-infection and ( c ) DBL-TH2 and -TH4 seropositive subjects at acute phase was follow up after recovery from infection for 3 years.

Article Snippet: Spots were count by Bioreader 5000 Pro-F gamma ELISPOT Reader (BioSys GmbH, Germany) (Supplementary Fig. ).

Techniques: Enzyme-linked Immunospot, Infection

Journal: Scientific Reports

Article Title: Persistence of Long-lived Memory B Cells specific to Duffy Binding Protein in individuals exposed to Plasmodium vivax

doi: 10.1038/s41598-018-26677-x

Figure Lengend Snippet: Long-lived DBL-TH-specific MBCs responses in individuals who had malaria attack for at least 3 years. MBC responses to a panel DBL-TH polymorphic strain were determined. PBMCs from 9 m and 3 y post-infection was used for ELISPOT assay. The scatter dot graph showing SFC-specific to DBL-TH2 and -TH4 per million stimulating PBMCs and the table showing association of plasma antibody response with ELISPOT specific to DBL-TH2 and -TH4 at ( a ) 9 m and ( b ) 3 y post-infection. HI refers to individual who had ≥ 80% inhibition activity of antibodies against DBL-TH-erythrocyte binding; LI refers to individual who had <80% inhibition activity. Tetanus toxoid-specific MBCs ( c ) was used as positive control of the experiment. The representative spot images are showed in Supplementary Fig. .

Article Snippet: Spots were count by Bioreader 5000 Pro-F gamma ELISPOT Reader (BioSys GmbH, Germany) (Supplementary Fig. ).

Techniques: Infection, Enzyme-linked Immunospot, Inhibition, Activity Assay, Binding Assay, Positive Control